Determining the accuracy of colorimetric pH testing compared to potentiometric methods

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Abstract
BACKGROUND: Bacterial growth in foods can be prevented by applying various controls to the food product, including adjusting the acidity of the food. Research has indicated that a pH level of 4.6 or lower will be effective to prevent most bacterial growth. In order to verify this level has been achieved pH test strips (colorimetric) or a digital calibrated pH meter (potentiometric) can be used. This study attempted to quantify the degree of accuracy that pH test strips have compared to the calibrated pH meter. METHOD: MColorpHastTM pH indicator strips with a pH range of 0-14 were tested against a calibrated Extech pH100 meter. In this study 40 samples of rice were acidified to varying levels. Each sample was measured with both colorimetric and potentiometric method. Results were compared to determine the level of accuracy of the pH test strips. As well, test strips were used to measure pH in a variety of different coloured preserves. RESULTS: A two-tailed test showed that there was a statistically significant difference between the readings from the pH test strips and the digital pH meter (P=0.0003). CONCLUSION: Based on the results, it can be concluded that both methods of measurement are not equally accurate. A calibrated pH meter will give more accurate readings of pH levels and should be used in most cases to confirm food safety with a high degree of confidence. In testing dark coloured jellies and preserves, pH test strips should not be relied on as they will be stained by the food, making the colorimetric reading difficult to determine accurately.

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Note
Project submitted in partial fulfillment of the requirement for the degree of Bachelor of Technology in Environmental Health, British Columbia Institute of Technology, 2017.
Degree granted
Bachelor of Technology (BTech) in Environmental Health
Publisher
British Columbia Institute of Technology
Number of pages
9 pages
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This license enables reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator. CC BY-NC-ND includes the following elements: BY: credit must be given to the creator. NC: Only noncommercial uses of the work are permitted. ND: No derivatives or adaptations of the work are permitted. https://creativecommons.org/licenses/by-nc-nd/4.0/